Fig. 3 | Scientific Reports

Fig. 3

From: The E3 ubiquitin ligase MARCH1 mediates downregulation of plasma membrane GABAB receptors under ischemic conditions by inhibiting fast receptor recycling

Fig. 3

MARCH1 is expressed in neurons and is upregulated under ischemic conditions. (a) All neurons tested expressed MARCH1. The expression of MARCH1 in neuron/glia co-cultures was tested by immunofluorescence staining using antibodies directed against NeuN (marker protein for neurons) and MARCH1 (scale bar: 50 μm). (b) MARCH1 is significantly upregulated after 1 h of OGD. Neuron/glia co-cultures were subjected to OGD for 30 min–1 h and tested for MARCH1 expression by immunofluorescence staining using antibodies directed against MARCH1. Cultures not treated with OGD served as a control. Top: representative images (scale bar: 10 μm). Bottom: quantification of fluorescence intensities (mean ± SD of 15 cells per condition, 2 independent experiments). One-way ANOVA followed by Dunnett’s multiple comparison test (ns, p > 0.05; **, p < 0.01).

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