Fig. 7
Treatment of OGD-stressed neurons with M1-Pep increased fast recycling of GABAB receptors and inhibited their targeting to the lysosomal pathway. Cultures were subjected to OGD for 1 h followed by treatment with M1-Pep. Neurons were analyzed about 16 h after OGD for the colocalization of GABAB receptors with endosomal markers by in situ PLA (upper panels) and for expression of the marker proteins (lower panels) using antibodies directed against GABAB2 and for the early endosome marker EEA1 (a), the fast recycling endosome marker Rab4 (b), the slow recycling endosome marker Rab11 (c), and the late endosome marker Rab7 (d). Scale bars, 10 µm. The data represent the mean ± SD of 25-30 neurons per condition derived from three independent experiments. One-way ANOVA followed by Tukey’s multiple comparison test (ns, p>0.05; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001).
