Fig. 3 | Scientific Reports

Fig. 3

From: Bone cell differentiation and mineralization in wild-type and osteogenesis imperfecta zebrafish are compromised by per- and poly-fluoroalkyl substances (PFAS)

Fig. 3

Larval bone formation following exposure to PFOS and PFOA in 6 dpf WT and Chi/+. (A) Representative images of PFAS exposed Sp7:GFP/WT and Sp7:GFP/Chi/+ operculum stained with alizarin red S. Scale bar: 50 μm. (B,C) Histogram representing the quantification of Sp7:GFP/WT and Sp7:GFP/Chi/+ operculum area delimited by osteoblasts normalized to the head area following PFOS (B) and PFOA (C) exposure. (D,E) Histogram representing the quantification of Sp7:GFP/WT and Sp7:GFP/Chi/+ mineralized operculum area normalized to the head area following PFOS (D) and PFOA (E) exposure. (PFOS: WT/Chi/+n ≥ 17, PFOA: WT/Chi/+n ≥ 14, the exact numbers are reported in Supplementary Table 4). Data are presented as mean ± SEM. Each point represents a single value, (Kruskal-Wallis rank sum test; * p < 0.05; ** p< 0.01). Control refers to not exposed fish.

Back to article page