Fig. 6

Lipid distribution following exposure to PFOS and PFOA in 6 dpf WT and Chi/+ larvae. (A) Representative image of Oil red O stained lipids. The spread of lipid droplets is qualitatively represented as moderate (1), intermediate (2) or diffuse (3) according to the distribution of lipids. (B) Representative image of Oil red O stained lipids and in 6 dpf WT and Chi/+ control and exposed to pollutants. Scale Bar: 100 μm. (C) Percentage of fish with 1, 2 or 3 levels of lipids spread in the liver. (PFOS: WT/Chi/+n ≥ 10, PFOA: WT/Chi/+n ≥ 8, the exact numbers are reported in Supplementary Table 4). Fisher’s exact test, * p < 0.05; ** p < 0.01 Control refers to not exposed fish.