Fig. 1
From: Recycling of endocytic BDNF through extracellular vesicles in astrocytes
Colocalization of endocytic BDNF with CD63 or Rab11. (A) Representative fluorescence images showing the colocalization of QD-BDNF with CD63- or Rab11-EGFP in astrocytes. QD-BDNF was added for 30–12 min to cultured astrocytes transfected with CD63-EGFP or Rab11-EGFP. Yellow arrowheads indicate QD-BDNF colocalized with the corresponding vesicular markers. Scale bar = 5 μm. (B) Bar graphs depict average colocalization ratios (number of colocalized QD-BDNF particles among total QD-BDNF particles). Dotted line: average colocalization ratio between QD-BDNF and MitoTracker (Mito.) as a negative control. *P < 0.05. N = 12–15 cells (194–2,782 QD particles). (C) Representative fluorescence images showing the colocalization of QD-BDNF with endogenous CD63 or Rab11 in astrocytes. Yellow arrowheads indicate QD-BDNF colocalized with the corresponding vesicular markers stained with anti-CD63 or anti-Rab11. Scale bar = 5 μm. (D) Bar graphs depict average colocalization ratios (number of colocalized QD-BDNF particles among total QD-BDNF particles). Dotted line: average colocalization ratio between QD-BDNF and MitoTracker (Mito.) as a negative control. *P < 0.05. N = 10–16 cells (784-2,110 QD particles).
