Fig. 3
From: Recycling of endocytic BDNF through extracellular vesicles in astrocytes
Knockdown of Vamp3 attenuated EV release from astrocytes. (A, B) Averaged distribution curves representing the concentrations of particles versus EV sizes isolated from the conditioned media of astrocytes transfected with either siSCR or siVamp3. Vehicle treatment was used to obtain spontaneously released EVs, while ATP treatment was used to obtain EVs released in response to ATP stimulation. *P < 0.05, **P < 0.01, ***P < 0.001. N = 5 batches. (C-E) Bar graphs showing the average number of particles categorized by size range. (C): 0.5–1000 nm, (D): 30–150 nm, (E): 150–1000 nm. *P < 0.05, **P < 0.01, ***P < 0.001. (F) Bar graphs showing the average particle size from each condition.
