Fig. 3
From: Loss of Kat2b impairs intraflagellar transport and the Hedgehog signaling pathway in primary cilia
The depletion of Kat2b impairs the recruitment of IFT and Hh components to primary cilia. (a, b) IFT components 6 h after serum starvation (early ciliogenesis). (a) Western blot data showed that protein levels of IFT components decreased in Kat2b silenced NIH/3T3 cells. (b) Fluorescence staining data revealed that the intensity of IFT components located in primary cilia was decreased in the stable Kat2b deleted cell. Scale bar: 2 μm (c-d) IFT components 24 h after serum starvation (maturated stage of ciliogenesis). (c) Western blot data show that protein levels of IFT components decreased in Kat2b silenced NIH/3T3 cells. (d) Immunocytochemistry data revealed that fluorescence intensity of IFT components located in primary cilia. Scale bar: 2 μm (e) Graphs showed fluorescence intensity of IFT25 and IFT52 under serum starvation 6 h, 24 h in stable Kat2b KO NIH3Te and control NIH3T3. (f) Immunocytochemistry data display that the fluorescence intensity of IFT25 and IFT52 (green) at primary cilia (red) in Kat2b KO MEF cells at 6 h and 24 h after serum starvation, respectively. Scale bar: 2 μm. (g) Graphs showed fluorescence intensity of IFT25 and IFT52 under serum starvation 6 h, 24 h in MEF cells. (h) Representative images displayed accumulation of IFT52 within cilia when treated garcinol and serum withdrawal 6 h, and below graph showed fluorescence intensity at cilia.
