Table 1 Analytical limits of detection (LOD) for RPA-Cas reactions.
From: Room temperature CRISPR diagnostics for low-resource settings
Cas12a | Temperature (°C) | LOD (copies)a | Figure | |
|---|---|---|---|---|
30 min | 60 min | |||
Lb | 20 | > 1,000,000b | > 1,000,000b | 2b |
25 | > 340,000c | 2300 (± 400) | S4 a, h | |
30 | 1400 (± 300) | 490 (± 80) | S4 b, h | |
37 | 196 (± 5) | 144 (± 2) | S3 c, h | |
Ts | 20 | > 150,000c | 740 (± 70) | S4 d, j |
25 | 290 (± 150) | 98 (± 7) | S4 e, j | |
30 | 165 (± 14) | 184 (± 1) | S4 f., j | |
37 | 89 (± 9) | 65 (± 1) | S4 g, j | |
