Fig. 6

Knockdown of PDHA1 can promote glycolysis in GBM cells. The PDHA1 expression in GBM cells (with GAMSC-EXO or not) was assessed by western blot (original blots data are presented in supplementary file) (A) and RT-PCR (B) ^*P < 0.05 vs. NC. The PDHA1expression in GBM cells (with siNC or siPDHA1 or not) was evaluated by western blot (original blots data are presented in supplementary file) (C) and RT-PCR (D) ^*P < 0.05 vs. siNC. ns: no sense. Statistical analysis of glucose utilization (E), lactate production rate (F), and intracellular ATP level (G) in the NC and siPDHA1 group, ^*P < 0.05 vs. NC. Oxygen consumption rate (H), basal respiration and maximal respiration analysis (I) of GBM cells in the NC and siPDHA1 group, ^*P < 0.05 vs. NC. Extracellular acidification rate (J), glycolysis and glycolytic capacity analysis (K) in the NC and siPDHA1 group, ^*P < 0.05 vs. NC.