Table 1 Metabolite alterations in the roots and shoots of salt-sensitive (IR28) and salt-tolerant (CSR28) rice seedlings were examined at two timepoints (6 h and 54 h) of high salt treatment.

From: Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance

	Root				Shoot
	6 h		54 h		6 h		54 h
	CSR28	IR28	CSR28	IR28	CSR28	IR28	CSR28	IR28
Amino acids
Alpha-Alanine	2.26 ± 0.09a	1.83 ± 0.18bc	1.10 ± 0.11d	− 1.27 ± 0.02e	− 1.08 ± 0.06de	− 1.49 ± 0.04e	1.96 ± 0.09b	1.60 ± 0.04c
Asparagine	3.15 ± 0.18cd	11.90 ± 0.28a	8.94 ± 0.76b	3.56 ± 0.32c	2.20 ± 0.07de	1.80 ± 0.27e	4.08 ± 0.22c	3.33 ± 0.25c
Aspartate	1.86 ± 0.06c	2.32 ± 0.08b	2.80 ± 0.12a	1.42 ± 0.05d	− 1.60 ± 0.02e	− 1.59 ± 0.02e	− 1.50 ± 0.01e	− 1.69 ± 0.05e
Beta-Alanine	2.72 ± 0.24cd	4.21 ± 0.35bc	8.08 ± 1.18a	3.14 ± 0.20cd	3.86 ± 0.15cd	9.10 ± 0.44a	5.48 ± 0.26b	2.43 ± 0.03d
Gaba	3.83 ± 0.25b	− 1.03 ± 0.19d	− 4.50 ± 0.01e	− 1.43 ± 0.03de	− 9.09 ± 0.01e	1.98 ± 0.31c	1.81 ± 0.09c	8.05 ± 0.33a
Glycine	1.19 ± 0.02d	1.48 ± 0.13cd	2.63 ± 0.16a	1.34 ± 0.09cd	− 1.13 ± 0.03e	1.52 ± 0.14c	1.91 ± 0.09b	− 1.78 ± 0.02f.
Glutamate	1.53 ± 0.02b	1.70 ± 0.06a	1.24 ± 0.03c	1.17 ± 0.03cd	− 1.03 ± 0.03e	− 1.07 ± 0.04e	1.01 ± 0.02e	1.13 ± 0.05d
Isoleucine	18.39 ± 1.31c	25.43 ± 1.56b	18.80 ± 1.32c	4.33 ± 0.36e	20.77 ± 0.60bc	10.59 ± 1.40d	42.86 ± 2.31a	25.17 ± 2.40b
Leucine	12.49 ± 0.69d	24.86 ± 4.06bc	9.80 ± 0.73d	3.01 ± 0.14e	21.54 ± 1.36c	14.71 ± 1.06d	31.06 ± 1.64a	27.38 ± 1.16ab
Lysine	8.14 ± 0.40b	9.60 ± 0.43b	16.03 ± 1.89a	2.87 ± 0.11c	15.41 ± 0.76a	13.67 ± 1.31a	15.01 ± 0.88a	6.91 ± 0.36b
Methionine	10.49 ± 1.05a	6.89 ± 0.43b	7.51 ± 0.91b	2.06 ± 0.16e	4.57 ± 0.17cd	3.88 ± 0.32cd	5.22 ± 0.11c	3.02 ± 0.36de
Phenylalanine	3.44 ± 0.21e	4.82 ± 0.13d	8.05 ± 0.13c	4.84 ± 0.09d	10.93 ± 0.25b	8.31 ± 0.18c	16.93 ± 0.29a	10.18 ± 1.20b
Proline	4.47 ± 0.30d	3.81 ± 0.28d	12.93 ± 0.81b	14.45 ± 1.2b	8.69 ± 0.46c	3.60 ± 0.26d	36.05 ± 2.11a	15.39 ± 1.22b
Putrescine	− 1.80 ± 0.02cd	− 1.01 ± 0.03b	− 1.82 ± 0.06cd	− 2.26 ± 0.04d	− 1.81 ± 0.05cd	− 1.46 ± 0.03c	1.16 ± 0.06a	− 1.02 ± 0.04b
Serine	4.84 ± 0.47c	8.65 ± 0.55a	5.93 ± 0.32b	3.80 ± 0.20d	2.14 ± 0.05e	1.95 ± 0.09e	5.21 ± 0.16bc	5.33 ± 0.36bc
Threonine	4.32 ± 0.47c	7.31 ± 0.36a	7.77 ± 0.31a	4.48 ± 0.58c	2.63 ± 0.08d	2.24 ± 0.17d	5.76 ± 0.20b	3.03 ± 0.16d
Tyrosine	2.82 ± 0.09d	3.32 ± 0.13cd	2.91 ± 0.22d	1.20 ± 0.07e	6.99 ± 0.21a	5.27 ± 0.51b	6.43 ± 0.26a	3.96 ± 0.31c
Valine	5.50 ± 0.17e	9.23 ± 0.87cd	12.10 ± 1.19b	2.28 ± 0.21f.	8.41 ± 0.41d	4.44 ± 0.36e	16.39 ± 0.55a	10.41 ± 0.34bc
Sugars and sugar alcohols
Glucose	1.05 ± 0.03c	2.25 ± 0.17a	− 4.13 ± 0.02f.	− 1.89 ± 0.04e	− 1.29 ± 0.01d	1.44 ± 0.07b	1.32 ± 0.09b	2.05 ± 0.02a
Glycerol	1.13 ± 0.06b	1.22 ± 0.08ab	− 1.75 ± 0.02e	− 1.34 ± 0.04d	− 1.04 ± 0.07c	1.36 ± 0.04a	1.34 ± 0.03a	1.21 ± 0.05ab
Fructose	− 1.34 ± 0.02c	− 1.68 ± 0.02d	− 11.11 ± 0.01e	− 6.76 ± 0.02e	− 1.27 ± 0.03c	1.36 ± 0.09b	1.41 ± 0.06b	1.84 ± 0.07a
Myoinositol	1.04 ± 0.02c	1.02 ± 0.01c	1.79 ± 0.05a	1.23 ± 0.04b	− 1.28 ± 0.03e	− 1.10 ± 0.02d	− 1.13 ± 0.02d	− 1.12 ± 0.04d
Raffinose	18.12 ± 0.28d	26.93 ± 0.94c	45.24 ± 4.00a	40.39 ± 1.3b	2.55 ± 0.17e	6.09 ± 0.51e	2.32 ± 0.15e	3.00 ± 0.12e
Organic acids
Alpha-Ketoglutarate	− 2.16 ± 0.02cd	− 1.79 ± 0.04c	− 3.42 ± 0.02d	− 1.81 ± 0.04c	− 1.12 ± 0.03b	2.75 ± 0.15a	− 1.67 ± 0.03c	1.03 ± 0.06b
Citrate	1.28 ± 0.04c	1.63 ± 0.07c	3.49 ± 0.19b	8.44 ± 0.26a	− 2.65 ± 0.02d	− 1.74 ± 0.04d	− 1.75 ± 0.03d	− 1.81 ± 0.01d
Fumarate	− 2.02 ± 0.04de	− 2.31 ± 0.03e	− 2.42 ± 0.03e	− 1.75 ± 0.03cd	− 1.60 ± 0.04bc	− 2.26 ± 0.02e	− 1.27 ± 0.04a	− 1.46 ± 0.05ab
Glycerate	1.23 ± 0.09b	1.43 ± 0.15a	1.22 ± 0.03b	1.03 ± 0.04c	− 3.65 ± 0.01f.	− 1.56 ± 0.02de	− 1.95 ± 0.01e	− 1.28 ± 0.03d
Glycolate	− 1.69 ± 0.05b	− 1.45 ± 0.07b	1.17 ± 0.14a	− 1.75 ± 0.04b	− 1.49 ± 0.04b	1.12 ± 0.10a	1.30 ± 0.11a	1.11 ± 0.04a
Hydroxyglutarate	4.92 ± 0.52a	1.33 ± 0.06bc	1.56 ± 0.06b	− 1.49 ± 0.05d	− 1.20 ± 0.03cd	− 1.08 ± 0.05cd	1.14 ± 0.04bcd	− 1.07 ± 0.04cd
Isocitrate	1.07 ± 0.02c	1.26 ± 0.09c	2.56 ± 0.14b	4.13 ± 0.09a	− 1.92 ± 0.03d	− 1.43 ± 0.08d	− 2.01 ± 0.02d	− 1.71 ± 0.05d
Lactate	1.04 ± 0.15ab	− 1.05 ± 0.13abc	− 1.05 ± 0.06abc	− 1.08 ± 0.03abc	− 1.37 ± 0.06c	− 1.10 ± 0.03abc	− 1.23 ± 0.05bc	1.20 ± 0.13a
Malate	− 1.85 ± 0.01c	− 1.32 ± 0.04a	− 2.66 ± 0.03e	− 1.20 ± 0.04a	− 2.06 ± 0.01cd	− 1.59 ± 0.03b	− 2.50 ± 0.02e	− 2.21 ± 0.01de
Malonate	−	−	−	−	− 2.16 ± 0.02b	− 2.18 ± 0.01b	− 2.31 ± 0.02b	− 1.95 ± 0.01a
Pyruvate	− 1.24 ± 0.03c	− 1.04 ± 0.08b	− 1.42 ± 0.02cd	− 1.68 ± 0.07d	− 1.64 ± 0.03d	1.05 ± 0.06ab	− 1.52 ± 0.03cd	1.11 ± 0.04a
Quinate	− 1.37 ± 0.06c	− 1.13 ± 0.02b	1.01 ± 0.05a	− 1.38 ± 0.03c	− 3.21 ± 0.00d	− 3.16 ± 0.02d	− 4.90 ± 0.01e	− 3.47 ± 0.01de
Shikimate	1.12 ± 0.02b	1.57 ± 0.16a	1.36 ± 0.09a	1.44 ± 0.11a	− 2.15 ± 0.01c	− 2.02 ± 0.03c	− 3.55 ± 0.01c	− 3.21 ± 0.02c
Succinate	− 1.56 ± 0.02c	− 1.16 ± 0.03b	− 1.71 ± 0.03c	− 1.56 ± 0.03c	− 1.07 ± 0.02b	1.47 ± 0.07a	− 1.07 ± 0.05b	− 1.08 ± 0.04b

The data is presented as Mean ± SE of salt/control ratios from five biological replicates. Ratios below 1 were inverted and multiplied by − 1. Values that are bold and underlined indicate no significant differences. Mean comparisons were performed using Duncan’s method (P < 0.05), with different letters representing each row.

Back to article page

Table 1 Metabolite alterations in the roots and shoots of salt-sensitive (IR28) and salt-tolerant (CSR28) rice seedlings were examined at two timepoints (6 h and 54 h) of high salt treatment.

Search

Quick links