Fig. 2

Dose-response curves for Triton X-100 and melittin treated microtissues. (a) Live/Dead images were captured for HepG2 microtissues treated with melittin at increasing concentrations. Multiple microtissues were imaged for every gel. Green: Calcein AM (live), red: ethidium homodimer (dead). Scale bar 1 mm. (b) A logistic-fitted, dose-response curve (back lines) for Triton X-100 treated microtissues were made using a nonlinear mixed effects model (NLME). Each error bar represents the 95% confidence interval from 20 individual spheroids for HepG2, MCF7, and cortical microtissues (n = 20 microtissues per condition; 3–4 independent experiments in different colors; HepG2 N = 620; MCF7 N = 800; neurospheroid N = 600). All data was normalized by the control group. The black lines and shaded gray regions surrounding them represent the NLME fit and its 95% confidence interval. (c) Lethal concentrations (LC) of Triton X-100 were estimated from the NLME line fit. Line traces delineate LC25 (blue), LC50 (red), and LC75 (yellow) concentrations. (d) Dose-response curves and NLME fitting for melittin treated microtissues (n = 20 microtissues per condition; 3–4 independent experiments; HepG2 N = 600; MCF7 N = 800; neurospheroid N = 600) were generated in the same manner as shown in (b). (e) LCs for melittin were generated in the same manner as shown in (c).