Fig. 4

Dose-response curves for paclitaxel treated microtissues. (a) HepG2 microtissues were treated with increasing concentrations of paclitaxel. The mean fluorescent intensity of tagged EdU with Cy5 (yellow) and nuclei with Hoechst (blue) were quantified. Scale bar 1 mm. (b) Dose-response curves were generated by quantifying the change in EdU expression with respect to Hoechst. The curves were fit using a nonlinear mixed effects (NLME) model using a logistic function (black lines). Each error bar represents the 95% confidence interval from 17–20 individual microtissues (n = 17–20 microtissues per condition; 3–4 independent experiments in different colors; HepG2 n = 19–20, N = 794; MCF7 n = 17–20, N = 796; neurospheroid n = 20, N = 580). All data was normalized by the control group. The black lines and gray shaded regions surrounding them represent the NLME fit and its 95% confidence interval. (c) Lethal concentrations (LC) values for paclitaxel were estimated from the NLME line fit. Line traces delineate LC25 (blue), LC50 (red), and LC75 (yellow) concentrations.