Fig. 5

Linear mixed effects model regressions for all assay-treatment pairs in HepG2 microtissues. HepG2 microtissues were treated with various compounds (columns) at the previously determined lethal concentrations and analyzed using four “gold-standard” assays (rows). Error bars present the 95% confidence intervals. Data was normalized to their respective controls then fitted to a linear regression using a linear mixed effects model (LME, black lines; shaded gray regions represent 95% confidence intervals). A dose-response was considered substantial when the p-value of the LME fit was p < 0.05 and the absolute value of the slope was greater than 0.1 (dark gray). ATP, Live/Dead, Caspase, and EdU assay datasets contained n = 8, 20, 8, or 20 microtissues per concentration over 2, 3, 2, or 2 independent experiments (in different colors), respectively. 2DG: 2-Deoxy-D-glucose, Oli: oligomycin A, Tri: Triton X-100, Mel: melittin, Cis: cisplatin, Mef: melphalan, Pac: paclitaxel. See Supplementary data, Figs. S1–S4 for analysis of MCF7 and cortical microtissues.