Table 1 Lethal concentrations (LC) resulting in 25, 50, and 75% cell death utilizing the “gold-standard” assays were reported alongside the multi-mechanism lethal concentration 50 (MMLC50). The MMLC50 thresholds were derived from principal component analysis run on the multimodal dataset. BPL: beyond practical limit, NA: not applicable, L/D: Live/Dead assay, neuro: Neurospheroid.
From: Multi-assay assessment of cytotoxicity reveals multiple mechanisms of action in 3D microtissues
2DG [mM] | Oligomycin [µM] | Triton X-100 [%] | Melittin [µM] | Cisplatin [µM] | Melphalan [µM] | Paclitaxel [µM] | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ATP | MMLC50 | ATP | MMLC50 | L/D | MMLC50 | L/D | MMLC50 | Caspase | MMLC50 | Caspase | MMLC50 | EdU | MMLC50 | ||
HepG2 | LC25 | 15 | 242 | 0.0034 | 2.8 | 11 | 31 | 37 | |||||||
LC50 | 52 | 55 | BPL | NA | 0.0054 | 0.0034 | 5.6 | 5.5 | 12 | 12 | 34 | 29 | 81 | NA | |
LC75 | 181 | BPL | 0.0090 | 11.1 | 14 | 38 | BPL | ||||||||
MCF7 | LC25 | 3 | 0.002 | 0.0023 | 3.5 | 120 | 48 | 0.0010 | |||||||
LC50 | 29 | 21 | 0.53 | 0.023 | 0.0050 | 0.0046 | 7.1 | 3.4 | 160 | 106 | 58 | 46 | 0.041 | 0.14 | |
LC75 | 354 | 190 | 0.0110 | 14.4 | 214 | 70 | 1.4 | ||||||||
Neuro | LC25 | 47 | 0.008 | 0.0034 | 0.30 | 5 | 18 | 20 | |||||||
LC50 | 110 | 96 | 0.058 | 0.011 | 0.0059 | 0.0043 | 0.60 | 0.36 | 7 | 5 | 22 | 17 | BPL | NA | |
LC75 | 265 | 0.455 | 0.010 | 1.2 | 10 | 27 | BPL | ||||||||
