Fig. 2

PS-NMPs exposure promotes liver fibrosis. (A) Representative images of MTS of liver tissue and (B) semi-quantitative results of liver fibrosis. Scale bar, 100 μm. (C) The mRNA level of α-sma was analyzed by qRT-PCR and normalized by β-actin (n=6). (D) α-SMA protein level was analyzed by western blotting and normalized by β-actin (n=5). (E) Representative images of IHC staining for α-SMA and (F) semiquantification of α-SMA based on IHC staining (n=8). Scale bar, 50 μm *p < 0.05, **p < 0.01, ***p < 0.001.