Fig. 2

Transcriptomic analysis indicates upregulation of inflammatory signatures in RK-derived compared to TC-derived CLL. a Principal component analysis of transcriptome from CD19⁺CD5⁺ CLL cells isolated from diseased TC (TC^CLL, n = 3) and RK mice (RK^CLL, n = 4), along with CD19⁺ B cells from aged-matched WT mice (n = 3). b Gene set enrichment analysis (GSEA) analysis showing hallmarks enriched in splenic B1a cells isolated either from diseased TC (TC^CLL, n = 3) or RK mice (RK^CLL, n = 4) compared to CD19⁺ B cells from aged-matched WT mice (n = 3). Hallmarks and NES (normalized enrichment score) are shown, if false discovery rate (FDR) ≤ 0.05 for TC or RK comparison. c Gene set enrichment analysis (GSEA) analysis showing hallmarks enriched in splenic B1a cells isolated from diseased TC mice (TC^CLL, n = 3) compared to aged RK mice (RK^CLL, n = 4). NES, normalized enrichment score; FDR, false discovery rate.