Fig. 4

Initial B1/CLL Expansion in RK-BL^KOmice. a Breeding scheme for generating RK-BL mice. Scheme was generated with BioRender.com. b Macroscopic appearance of representative spleens (SP), inguinal lymph nodes (ilN) and mesenteric lymph nodes (mLN) from six-month-old RK-BL^KO and BL^KO mice. c Dot plot graph depicts spleen (SP) weight in gram (g) of six-month-old mice with indicated genotypes (n = 3–10). d Splenic cell count of living cells isolated from six-month-old mice with indicated genotypes (n = 3–8). e Dot plot graph depicts mesenteric lymph node (mLN) weight in gram (g) of six-month-old mice with indicated genotypes (n = 3–10). f Cell count of living cells isolated from the mesenteric lymph nodes from five- to six-weeks old mice with indicated genotypes (n = 2–9). g Percentages of CD19⁺CD5⁺ cells of living splenocytes isolated from six-month-old RK-BL^KO mice (n = 8) and control mice (n = 3–7). h Percentages of marginal zone (MZ) B cells (defined by CD21⁺C23^neg expression) pre-gated on CD19⁺CD5^neg splenocytes isolated from six-month-old RK-BL^KO mice (n = 8) and age-matched control mice (n = 3–7). i Percentages of follicular (FO) B cells (defined by CD21^lowCD23⁺ expression) pre-gated on CD19⁺CD5^neg splenocytes isolated from six-month-old RK-BL^KO mice (n = 8) and age-matched control mice (n = 3–7). Statistical analysis was performed using one-way ANOVA with Tukey correction for multiple comparison. P values are indicated in respective graphs. All data are presented as mean ± standard deviation.