Fig. 1
ELT inhibited the proliferation of PLC/PRF/5 and HUH7 cells without causing cell death. (A) The molecular structure of ELT. (B) The cell growth inhibition of ELT at various concentrations for 24, 48, and 72 h, respectively, by MTT assay. (C) Images of the colony formation following a 14-day exposure to various concentrations of ELT. (D) Images of cell cultures after a 48 h ELT treatment followed by an EdU proliferation assay. Scale bars, 100 μm. (E) The protein level of PCNA in two cell lines after being treated for 48 h with ELT at various concentrations by Western Blotting. Original blots were presented in Supplementary Fig S3. (F) The Annexin V/PI staining of cells after treating with ELT at 8 µM in 6-well plates for 48 h. (G-I) The statistical analyses of the EdU positive cells, protein level of PCNA, and apoptosis cell rate. The data derived from three separate experiments were displayed as the average ± SD, *, p < 0.05, ns, p > 0.05.
