Fig. 4
Expression and GO/KEGG enrichment analyses of tsRNA in ELT-treated HCC. (a) Clustering heatmap of the differentially expressed genes of ten tsRNAs in the ELT and control groups. Orange represented up-regulated tsRNAs, and blue represented down-regulated tsRNAs in the groups. (b) The volcano plot displayed tsRNAs between the ELT and control groups. The blue dots represented the down-regulated tsRNAs, and the red dots represented the up-regulated tsRNAs. | FC > 1.5 |, p-value < 0.05. (c) GO analyses of predicted target genes of tsRNAs. (d) KEGG, developed by Kanehisa Laboratories45, enriched the terms associated with the predicted target genes of tsRNAs in the ELT and control groups. (e) The diagram of 21 common genes at the intersection of Wnt and cancer pathways. These two pathways were referenced to Kanehisa et al.46. (f) The ranking chart of 21 common genes. (g) The relative expression level of tRF5-26-AlaAGC-13, 5’tiRNA-36-AlaAGC-10, tRF5-26-LysTTT-7, and 5’tiRNA-31-GluCTC-16 in PLC/PRF/5. U6 served as a control. (h, i) The level of DVL2 and DVL3 in two cell lines after a 48-h exposure to various concentrations of ELT by Western blotting. Tubulin served as a control. (j) The relative expression of DVL3 and DVL2 mRNA in PLC/PRF/5 cells after treating with tRF5-26-AlaAGC-13 or 5’tiRNA-36-AlaAGC-10 mimic. Results were expressed as mean ± SD of three independent experiments. *, p < 0.05; ns, p > 0.05. The samples derive from the same experiment, and the blots were processed in parallel. Original blots were presented in Supplementary Fig S6.
