Fig. 5
The characteristics of tRF5-26-AlaAGC-13. (A) tRF5-26-AlaAGC-13 was a 5’tRF fragment of tRNA-Ala-AGC, derived from three mature tRNA precursors (tRNA-AlaAGC-22-1, tRNA-AlaAGC-13-1, tRNA-AlaAGC-13-2). (B) The genomic location of tRNA-AlaAGC-22-1 was identified as chromosome 6p12.2, spanning a length of 73 bp, as shown in the UCSC Genome Browser database. (C) The genomic location of tRNA-AlaAGC-13-1 was identified as chromosome 6p22.2, spanning a length of 73 bp, according to the UCSC Genome Browser database. To view further details of an item, simply click on it; this was indicated by the red arrows. (D) tRNA-AlaAGC-13-2 was located on chromosome 6p11.2, with a length of 73 bp, using the UCSC Genome Browser database. Details of the item could be displayed by clicking on it, as pointed out by the red arrows. (E-G) tRF5-26-AlaAGC-13 was derived from tRNA-AlaAGC-22-1, tRNA-AlaAGC-13-1, and tRNA-AlaAGC-13-2, which had a length of 26 nt. The cleavage site was located at the end of the D-loop sequence (CGCT). (H) tRF5-26-AlaAGC-13 showed a match with DVL2 or DVL3 mRNA. The symbol “|” indicated a perfect match. “:” indicated that a T: G or G: U match was made. 8mer-1a meant 3’-UTR matched exactly with nucleotide 2–8 nt of 5’tRF, and 1a represented the complementary pairing position of UTR and tRF5’ where 1 nt was A. Local AU represented a region rich in AU, which was more likely to exert a biological function. The red bar indicated that the position was A: U, and that a greater degree of complementarity was preferable.
