Fig. 1

Tumoroid lines can be derived from donor material across several cancer indications. (a) Schematic of tumoroid establishment and characterization. Donor tumor material was processed, cultured, and analyzed for fidelity to the original cells sampled at the time of collection by next-generation sequencing. (b) Representative images of tumoroid lines at passages (P) 0, 5, and 10. P0 images were acquired after 1–2 weeks of culture. P0 and P5 images represent a mix of embedded and suspension samples. All P10 images were suspension cultures. HuCo = human colorectal cancer; HuLu = human lung cancer; HuEn = human endometrial cancer. Scale bar = 400 μm. (c) Cumulative population doublings (PD) over time during attempted derivation of colorectal, lung, endometrial, and breast tumoroid lines. Dashed lines indicate short-term cultures that formed tumoroids initially but failed to expand in vitro over the long term. (d) Time (in days) for successfully established tumoroid lines to reach 5 PD based on initial cell seeding number for various indications. Each point represents one donor, and each line is plotted at the median. For panels (c) and (d), successful colorectal donors – HuCo1044, HuCo3209, HuCo021320, HuCo031721, HuCo111622, HuCo080922, HuCo090123, HuCo083123, HuCo100523; successful lung donors – HuLu051421, HuLu051921; successful breast donor – HuBr102622; successful endometrial donors – HuEn033122, HuEn041521, HuEn061022, HuEn070722, HuEn022222, HuEn052622B, HuEn052622A, HuEn062322.