Fig. 4
From: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
OncoPro medium-derived tumoroids are stable during long-term culture. (a) Morphology and (b) growth rate of tumoroid lines during long-term culture. Cumulative population doublings (PD) were measured by cell counts at each passage. Tumoroid lines were recovered from cryopreservation at the beginning of this experiment (day 0), and additional cryopreservation and recovery points are indicated by arrows for each culture. (c) Pearson’s correlation of single nucleotide variants (SNVs; indicated by points) between tumor samples (P0) and tumoroids at bank and at late passage numbers with regression lines, confidence intervals, and density ellipses encompassing 90% of the data (top). Each dot represents the variant allelic frequency (VAF) for 1 genetic locus covered by the Oncomine Comprehensive Assay v3 (OCAv3). Single base substitutions (bar graphs, middle) and oncogenic mutations (heat maps, bottom) from multiple time points during this study. The oncogenic mutations were called using the Oncomine Variants filter 5.18 within the Ion Reporter Software.
