Fig. 6 | Scientific Reports

Fig. 6

From: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications

Fig. 6

Publicly available tumoroid lines can adapt well to culture in OncoPro medium. (a) Outline of study comparing growth rate of tumoroids from biobanks (for example, National Cancer Institute Patient-Derived Models Repository, PDMR) in homebrew medium to growth of tumoroids in OncoPro medium in embedded or suspension culture formats. (b) Tumoroid lines from colorectal, lung, breast, head and neck, and pancreas cancer cultured in PDMR-recommended homebrew media or in OncoPro medium in embedded or suspension culture have similar morphologies and growth rates. For model 868763-120-R-V1-organoid, cells were initially expanded in PDMR-recommended media for several passages to create a working bank and then cultured in all three conditions in parallel. Scale bar = 400 μm.

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