Fig. 8
From: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
Tumoroid suspension cultures are amenable to multiplexed assays for immune cell cytotoxicity studies. A colorectal tumoroid lineĀ (HuCo1044) stably expressing green fluorescent protein (GFP) was dissociated and seeded into 96 well microcavity plates. After 64Ā h, natural killer (NK) cells from a primary source or the NK-92 cell line were added at various effector to target (E: T) ratios along with an indicator of caspase 3/7 activity, Invitrogen CellEvent Caspase-3/7 Red. (a) Representative images of HuCo1044-GFP cells acquired on an Incucyte Live-Cell Analysis System 24Ā h after addition of primary NK cells at multiple E: T ratios (0:1 represents tumoroids only condition). Scale bar =ā800Ā Ī¼m. (b) Quantitative image analysis of green (HuCo1044-GFP) or red (caspase-3/7 activity) integrated fluorescence intensity (green or red calibrated unitsāĆāĀµm2/well) before and after addition of Primary NK or NK-92 cells at 64Ā h (dotted line). Plots show average intensity values across three replicate wells per condition.
