Fig. 3 | Scientific Reports

Fig. 3

From: Discovery of a sushi domain-containing protein 2-positive phenotype in circulating tumor cells of metastatic breast cancer patients

Fig. 3

Detection and validation of differentially expressed proteins between CTC-ITB-01 vs. MCF-7.A: Analysis of the mTOR pathway based on SILAC-based LC-MS/MS proteomic data. Synonyms: MAPKAP1: SIN1, MLST8: GβL, AKT1S1: PRAS40, RPS6KB1: P70S6K1. For detailed values of the protein identification and quantification, see Supplementary Table S3.B: Western Blot analysis of the denoted proteins (n = 3). BC-M1 was selected as an example of a disseminated tumor cell line from the bone marrow of a breast cancer patient. MDA-MB-468 was selected as an example of a triple negative breast cell line.C: Quantitative Western Blot analysis of PDCD4 (n = 3). The average value is shown as numbers and the standard deviation as vertical error bars. The p-value was calculated using Student’s two-sided t-test with p < 0.05 considered significant.D: Detection of PDCD4 in tumor cells of breast cancer patients (n = 3). The composite images show overlays of the DAPI, CD45, keratin and PDCD4 signals. Uncropped X-ray film images are shown in supplementary file F1.

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