Fig. 3
(A,C) Lysates from MIN6 cells were incubated with or without SY (50 μM) for 12 h and subjected to CETSA assay. SY protects the target protein against temperature-dependent degeneration in MIN6 cells. GOAT was normalized with GAPDH. Note that the membrane was cropped to remove irrelevant parts. (B,D) The depiction of CETSA after quantification using western blot. (E) Lysates from MIN6 cells were incubated with or without SY (50 μM) for 12 h. Different concentrations of pronase E (0, 0.01%, 0.03%, 0.1%, 0.3%, and 1%) were added for 30 min, and GOAT content was detected using western blot analysis. Note that the membrane was cropped to remove irrelevant parts. SY promoted the resistance of the target protein GOAT to proteases. (F) The depiction of DARTS after quantification using western blot analysis. All experiments were repeated three times independently. Statistical significance was determined by ANOVA with Dunnett’s test (B,D,F). Results are expressed as the mean ± SD, and *P < 0.05 versus control group.
