Fig. 5
Effects of SY on the GOAT-activated signaling pathway in HG-induced MIN6 cells. MIN6 cells were stimulated with or without SY (50 μM) for 12 h. (A) Protein levels in the GOAT signaling pathway. Note that the membrane was cropped to remove irrelevant parts. (B) cAMP levels in the medium were determined. (C) Quantitative analysis of the pathway proteins. All experiments were repeated three times independently. Statistical significance was determined by ANOVA with Dunnett’s test (B,C). Results are expressed as mean ± SD, and **P < 0.01, ***P < 0.001 versus control group.
