Fig. 6
Fluorescent amyloid ligand combinations for discrimination of amyloid structures in mouse tissue. (A) Hyper spectral fluorescence micrographs of AA mouse spleen co-stained with X-34 and HS-310. Amyloid is clearly visible. Regions of interest (ROI 5 × 5 pixels) were selected as indicated by arrows and emission spectra from each ROI are displayed in (B). White arrows are depict spectra (black lines) within the amyloid follicle rim, red is outside of follicle and green is inside. X-34 emits with a peak centered at 460–480 nm and HS-310 with a double peak in the interval 530–570 nm. (C) The fluorescence ratio of X-34 and HS-310 emission at 471/552 nm for ROIs from the different groups of mice (n = 3 in each group). Statistics in C: One-way ANOVA with Tukey’s test comparing all groups: *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001. ns nonsignificant.
