Table 4 Comparison of relative level of 16S rRNA before and after 4 h exposure to agents. Comparison of relative level of 16S rDNA before and after 4 h exposure to the agents.

From: Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols

Shed Biofilm Assay1

16 S rRNA Level2

p value vs. 0 h3

16Sr DNA Level

p value vs. 0 h

Vehicle + 0 h Incub.

1.0 ± 0.91

 

1.0 ± 0.51

 

Vancomycin + 4 h Incub.

 2.2±  1.24

0.012

2.43 ± 0.95

0.0658

Penicillin G + 4 h Incub.

 0.97 ± 0.58

0.67

1.57 ± 0.76

0.121

Cranberry + 4 h Incub.

. 0.63±  0.53

0.67

0.43 ± 0.38

0.0203

  1. 1. Assay time and agent exposed to shed biofilm.
  2. 2. Total 16S rDNA for each incubation is normalized to the level of that molecule at baseline prior to the 4 h incubation.
  3. 3. Mann Whitney Test used to assess significance of difference versus baseline value which is normalized to one.
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