Fig. 2 | Scientific Reports

Fig. 2

From: Metformin inhibits the growth of SCLC cells by inducing autophagy and apoptosis via the suppression of EGFR and AKT signalling

Fig. 2

Metformin blocks the cell cycle of SCLC cells. (A) The cell cycle distribution of SCLC cells was detected by flow cytometry. The cells were treated with different concentrations of metformin (0, 5, and 10 mM) for 48 h. The data are presented as the means ± SDs of values from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group. (B,C) The expression levels of cell cycle-related proteins were determined by western blotting in H446, H446/DDP (B), H526, and H526/DDP cells (C) after treatment with metformin (MTF). Pixel density analysis of protein expression compared with that in the untreated control groups (set to 1). Significant difference compared with the untreated control: *p < 0.05; **p < 0.01; ***p < 0.001. n = 3.

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