Fig. 1
From: Morphological and functional analysis of colorectal cancer cell lines in 2D and 3D culture models
E-cadherin and vimentin mRNA expression in 2D and 3D cultured colorectal adenocarcinoma (CRAC) cell lines. (a) E-cadherin and (b) vimentin mRNA levels were examined using real-time quantitative polymerase chain reaction (qRT-PCR). Results are presented as the means ± standard deviation (SD) from three independent experiments. The results are shown after normalization against the values obtained for COLO-320 cells under the 2D culture (value = 1). DLD-1, CACO-2, and HCT-15 cells had high E-cadherin and low vimentin mRNA levels, whereas SW480 and COLO-320 cells had low E-cadherin and high vimentin mRNA levels in the 2D culture (black bars). DLD-1 cells, which exhibited the highest E-cadherin expression in 2D culture, showed a significant decrease in E-cadherin expression in 3D culture. COLO-320 cells, which had the highest vimentin expression in 2D culture, and SW480 cells, which had the second-highest vimentin expression, both demonstrated significantly decreased vimentin expression in 3D culture (white bar). * p < 0.05.
