Fig. 7
From: Morphological and functional analysis of colorectal cancer cell lines in 2D and 3D culture models
Analysis of cell proliferation ability. (a) Immunocytochemical analysis of Ki-67 in CRC cells under 2D (upper row) and 3D (lower row) culture conditions. Ki-67-positive cells were diffusely present in cancer cells. Scale bar = 100 μm. (b) Quantification of Ki-67-positive cells. There were differences in the number of Ki-67 positive cells in 2D and 3D cultures for DLD-1, CACO-2, and HCT-15 cells, but no change in the number of Ki-67 positive cells in SW480 and COLO-320 cells. Data are shown as the mean ± standard deviation (SD). *p < 0.05.
