Table 2 The descriptive statistics of the raw Ct values of all tested reference gene candidates for the kidney and the liver.

From: Evaluation of reference genes for qPCR in human liver and kidney tissue from individuals with obesity

 

Kidney tissue (n = 28)

Liver tissue (n = 32)

Mean ± SEM

Median (Q1; Q3)

Min – max

Mean ± SEM

Median (Q1; Q3)

Min – max

ACTB

25.85 ± 0.43

25.71 (24.03; 26.76)

20.60–30.95

25.42 ± 0.31

25.32 (24.63; 26.49)

22.11–30.35

B2M

26.05 ± 0.42

25.93 (24.49; 27.29)

20.76–30.65

25.53 ± 0.34

25.61 (24.19; 26.67)

22.35–30.54

GAPDH

26.34 ± 0.41

26.18 (25.34; 27.36)

21.97–30.72

25.94 ± 0.25

25.89 (25.30; 26.67)

23.06–29.99

HPRT1

30.29 ± 0.32

29.93 (29.19; 31.26)

26.52–33.45

31.13 ± 0.26

30.91 (30.26; 31.90)

28.35–34.51

RPLP0

25.63 ± 0.40

25.61 (24.30; 26.66)

20.42–29.61

24.82 ± 0.24

24.68 (23.90; 25.64)

22.46–27.85

18S RNA

9.40 ± 0.09

9.40 (9.06; 9.71)

8.57–10.52

8.50 ± 0.07

8.47 (8.24; 8.70)

7.66–9.44

PPIA

30.85 ± 0.39

30.83 (28.96; 32.59)

27.06–34.21

29.56 ± 0.33

29.45 (28.52; 30.25)

25.81–34.24