Fig. 2 | Scientific Reports

Fig. 2

From: Extracellular vesicles in seminal plasma of Sahiwal cattle bulls carry a differential abundance of sperm fertility-associated proteins for augmenting the functional quality of low-fertile bull spermatozoa

Fig. 2

Characterization of extracellular vesicles. (A) Intensity-based size distribution of seminal plasma-derived EVs, as assessed by the Zetasizer nano zs particle sizer. Each curve shows means ± SD from three replicates in a representative experiment out of the three performed with similar results. (B) Nanoparticle Tracking Analysis (NTA) on the buffalo blood plasma-derived small EVs under 100 × dilutions. Finite track length adjustment (FTLA) size per concentration graph, taken as five replicates, the graph represents the averaged FTLA size per concentration (particles/mL) (C) Transmission Electron Microscopy (TEM). TEM image of EVs derived from Seminal plasma. EVs were negatively stained with 1% phosphotungstic acid after removing the extra moisture. (Magnification-100000X and 150000X, Scale bar—50 and 100 nm, 120 kV) (E) Identification of the TSG 101, CD63 EV-specific protein markers and Calnexin EV negative marker by the western blot analysis of isolated pooled EVs samples from the seminal plasma of sahiwal bulls. Full blot images are shown in Supplementary Fig. S3A–C.

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