Fig. 4

Uptake of PKH26 labeled SPEVs by the spermatozoa visualized by fluorescence microscopy. SPEVs and nucleus were stained with red and blue respectively. The images were captured at 600X magnification (A, D) spermatozoa stained with nuclei stain (DAPI-Blue). (B, E) Spermatozoa stained with lipophilic dye (PKH26 -Red). (C, F) Merged images of DAPI and PKH 26 labeled spermatozoa.