Fig. 6 | Scientific Reports

Fig. 6

From: Extracellular vesicles in seminal plasma of Sahiwal cattle bulls carry a differential abundance of sperm fertility-associated proteins for augmenting the functional quality of low-fertile bull spermatozoa

Fig. 6

Fluorescent microscopic images of LF spermatozoa after incubation with SPEVs of HF Sahiwal Bulls and control. (A) live Un-capacitated pattern (F, bright green fluorescence distributed uniformly over entire sperm head, with or without stronger fluorescent line at the equatorial segment), (B) live capacitated pattern (B, green fluorescence over the acrosome region and a dark post acrosome), (C) live acrosome-reacted pattern (AR, live acrosome-reacted pattern, no fluorescence above the head) (D) Histogram representing the capacitation status of the LF spermatozoa upon incubation with HF SPEVs.

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