Fig. 7 | Scientific Reports

Fig. 7

From: Extracellular vesicles in seminal plasma of Sahiwal cattle bulls carry a differential abundance of sperm fertility-associated proteins for augmenting the functional quality of low-fertile bull spermatozoa

Fig. 7

Overlay of the MFI histograms obtained by flow cytometry analysis of LF spermatozoa upon EV incubation of HF bulls at different dilutions with (A) FITC-PNA and (B) Mitosox stain. (n = 3) each group. (C) Average MFI histogram of acrosome-reacted sperm in an LF bull after incubation with SPEVs at different dilutions, i.e., 1X, 2X, 4X, 8X, and 16X. (D) Average MFI histogram of ROS production in spermatozoa of LF bulls after incubation with SPEVs at different dilutions in PBS, i.e., 1X, 2X, 4X, 8X, and 16X.

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