Table 1 List of supplements and growth factors used in HN PDO culture medium​.

From: Head and neck tumor organoid grown under simplified media conditions model tumor biology and chemoradiation responses

 

Supplements/growth factors

Vendor

Catalog #

Final concentration

Reagent A

1

Bovine Pituitary Extract (BPE)

Hammond cell tech

1078-NZ

0.8 mL for 100 mL

2

B27

Thermo

17,504,001

1.0 mL for 100 mL

3

Recombinant Human FGF- Basic (FGF2)

Peprotech

AF-100- 18B

10 ng/mL

4

Recombinant Human FGF10

Peprotech

100 − 26

10 ng/mL

5

Recombinant Human EGF

Peprotech

AF-100-15

10 ng/mL

6

Recombinant Human IL6

Peprotech

200-06

100 ng/mL

7

Recombinant Human Amphiregulin

Peprotech

100-55B

50 ng/mL

8

Recombinant Human Prolactin

Peprotech

100-07

10 ng/mL

9

Human Insulin

Sigma

I2643- 250MG

10 µg/mL

10

Cholerae Toxin

Sigma

C8052-2MG

200 ng/mL

Reagent B

1

Hydrocortisone

Sigma

1316004-200MG

0.5 µg/mL

  1. The table specifies each reagent’s name, vendor, catalog number, and final concentration used. These reagents support the growth and differentiation of the head and neck cancer organoids in vitro, providing a conducive environment for tumor modeling.
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