Fig. 1
From: Dissecting the sterility phenotype in gene edited Drosophila suzukii pgSIT males
pgSIT Drosophila suzukii males are 100% sterile. (a) A schematic of the gRNAsxl, βtub construct used to generate the gRNA line. This line is tagged with a green-fluorescent genetic marker. (b) Schematic representation of the vasCas9 construct used to generate the Cas9 line. This line is tagged with a red fluorescent genetic marker. (c) The crossing scheme used to assess the fertility of males from wildtype, vasCas9, gRNAsxl, βtub, and pgSIT strains. Males from each strain were separately outcrossed to wildtype females. Progeny from these crosses were used to assess fertility. (d) pgSIT males are completely sterile compared to wildtype, vasCas9, and gRNAsxl, βtub outcrosses. No significant differences were observed when comparing vasCas9 and gRNAsxl, βtub offspring data to wildtype. The average number of offspring for each strain is plotted as colored bars. The colored circles indicate the number of offspring in each replicate. The red vertical bars indicate ± SEM. Statistical significance of means was evaluated using unpaired t-test. (p > 0.05ns, p < 0.0001****).
