Fig. 1
BMDA treatment diminishes ROS production and DNA damage induced by UVB irradiation. (A) HaCaT cells were pre-incubated with folic acid (10 µM) or BMDA addition (1–2 µg/mL) 2 h before UVB radiation. HaCaT cells were exposed to UVB (48 mJ/cm2) after removing the medium and then cultured for 18 h under folic acid (10 µM) or BMDA addition (1–2 µg/mL). DCF-DA (25 µM) was then added to the medium for 20 min, and the production of ROS was detected under fluorescence microscopy. Three images were captured randomly, and the fluorescence intensity was analyzed using Victor3. (###p < 0.001, against “Untreated” group, ***p < 0.001, against “DMSO” group) (B) The cells under the same condition were harvested after UVB exposure for the detection of γ-H2AX using immunoblotting. The cell lysates were separated at 10% SDS-PAGE. γ-H2AX protein was detected with its antibody after transferring the gel to a nitrocellulose membrane. γ-H2AX protein was relatively quantified after scanning of the gel. (#p < 0.05, against “Untreated” group, *p < 0.05, against “DMSO” group)
