Fig. 3
BMDA treatment relieves UVB-induced cell cycle arrest. HaCaT cells were pre-incubated with folic acid (10 µM) or BMDA addition (1–2 µg/mL) 2 h before UVB radiation. HaCaT cells cultured on slide chambers were irradiated with UVB (48 mJ/cm2) and cultured for 18 h under folic acid (10 µM) or BMDA addition (1–2 µg/mL). The cells were fixed and permeabilized with 70% cold ethanol for 1 h and resuspended with PI (100 µg/mL). RNase (100 µg/mL) was added for 30 min, and the cell cycles thereafter were analyzed using FAM. Three independent experiments were performed, and the representative FACS data were displayed. (***p < 0.001, against “DMSO” group)
