Fig. 5
Topical application of BMDA prevents wrinkle formation on the skin induced by repeated UVB exposure. (A) Wrinkle formation on the skin under repetitive UVB irradiation was measured by replica grading after four weeks of BMDA treatment. Wrinkles were evaluated as described previously29 (grade 0, no wrinkles; grade 1, a few shallow wrinkles; grade 2, some wrinkles; grade 3, several deep wrinkles). (**p < 0.01, ***p < 0.001, against “Control” group) (#p < 0.05, ###p < 0.001, against “Cream” group) (B) The dorsal skin tissues irradiated by UVB for four weeks were taken and sonicated with RIPA buffer containing 0.1% SDS. After centrifugation, the cell lysates were separated at 6% SDS-PAGE. Collagen I protein was detected with its antibody after transferring the gel to a nitrocellulose membrane.
