Fig. 8 | Scientific Reports

Fig. 8

From: N-benzyl-N-methyldecane-1-amine derived from garlic ameliorates UVB-induced photoaging in HaCaT cells and SKH-1 hairless mice

Fig. 8

BMDA treatment reduces protein levels of TNF-α and IL-1β in the skin tissue. Paraffin was removed from paraffin-embedded tissue sections and the tissue was then rehydrated. Tissue antigens were exposed after boiling with unmasking solution and incubated with anti-TNF-α or IL-1β antibody. The fluorescence image was observed under an immunofluorescence microscopy after staining with FITC-conjugated secondary antibodies.

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