Fig. 3

Characteristics of collective migration dynamics. a Representative example of dynamics of collective cell migration change upon dex treatment. Dex treatment initially—in the first 6 h—inhibits edge rate compared to the control. b Egde rate was higher in the 1st hour of treatment in vehicle (DMSO) controls, while it was balanced after the 6th hour of treatment. c Edge rate after 6 h could not be reliably evaluated due to the low rate (0.01 μm/s), which is a limitation of our instrument. However, wound sizes indicated a dex-induced migration-promoting effect compared to the controls: d Representative example of wound closure upon dex treatment. e-f Wound size significantly reduced in the dex treated condition after the 4-6th hour of treatment compared to controls. Columns represent the averages of four biological replicates, with error bars indicating standard deviations. For statistical comparison of DMSO- and dex-treated conditions, in each time points they were compared using unpaired t-test. ns: p > 0.05; *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001; ****: p ≤ 0.0001.