Fig. 4

Characteristics of single-cell migration dynamics. a Representative image showing single-cell migration distances. Variability in migration distance was observed across individual cells in both dex- and vehicle (DMSO)-treated groups; however, dex treatment increased the accumulated migration distance, as indicated by the differently scaled axes. b Accumulated migration distance was influenced by cell seeding density but increased independently of seeding density under dex treatment. c-f During the first hour of treatment, vehicle (DMSO)-treated cells showed faster migration compared to later hours, while the migration-promoting effect of dex became evident as early as the second hour. Data in panels c-f represent the average migration distances of 35 control (DMSO) and 40 dex-treated cells tracked across three experiments. Statistical comparisons between DMSO and dex treatments at each time point were conducted using an unpaired t-test. ns: p > 0.05; ****: p ≤ 0.0001.