Fig. 5

a Gene expression changes following dex treatment. Statistical comparisons between DMSO and dex-treated conditions at each time point were performed using an unpaired t-test. b NR3C1 expression levels varied across the four cell lines, with the lowest expression observed in T47D cells. Statistical significance was assessed by ANOVA followed by Tukey’s test for multiple comparisons. ***: p ≤ 0.001; ****: p ≤ 0.0001. Columns in panels (a-b) represent the averages of three biological replicates, with error bars showing standard deviations. c Heatmap illustrating the global transcriptomic profile of breast cancer cell lines treated with vehicle control (DMSO) and dex. d Heatmap of differentially expressed genes between DMSO- and dex-treated conditions. “up” and “down” indicate genes commonly up- or down-regulated across cell lines, while “alt” marks genes with treatment-specific expression differences among the cell lines.