Fig. 8

GR gene expression and GR signature in association with migration in fast and slow-migrating triple-negative single breast cancer cells. The panels depict reanalysis of single-cell sequencing data from three studies: Pal et al. 2021 (PMID: 33950524; panels a–g), Bassez et al. 2021 (PMID: 33958794; panels h–o), and Wu et al. 2021 (PMID: 34493872; panels p–v). a, h, p: Spearman correlation between GR signature and migration signature at the single-cell level across the three studies. b: UMAP visualization of gene expression patterns in single triple-negative breast tumour tissue cells of the study of Pal et al. 2021. generated using a dimensionality reduction algorithm. Based on cell-specific marker expression (see Supplementary Fig. 1), breast cancer cells were grouped into eight clusters (0, 1, 2, 8, 13, 14, 15, 18), highlighted by the red circle (other cell types are shown in separate clusters). These tumour cell clusters were further analysed on panel (c-g). Representative images of migration (c , j , r) and GR signature (d , l , s), NR3C1 (e , m , t), and GR target SGK1 (f , n , u) and TSC22D3 (g , o , v) gene expression in different tumour cell clusters. The migration signature between individual tumour cells with low and high GR expression were compared using the Wilcoxon test (i , q).