Fig. 1
Effect of repeated administration of nicotine on the induction of hyperlocomotor activity and oxidative stress via the generation of ROS in the NAc of rats. (A). Experimental timeline for the measurement of locomotor activity (a) and the assessment of ROS production and oxidative stress marker expression in the NAc of rats (b). B, C. Mean total distance traveled on days 1, 3, 5, and 7 and representative locomotor trajectories (red lines in each gray rectangle) on day 7 (B), and mean total distance traveled at each time period after saline or nicotine administration on day 7 (C). *P < 0.05, ***P < 0.001 vs. the saline group, ##P < 0.01 vs. the nicotine group on day 1. n = 5/group. D, E. Measurement of ROS levels (D) and representative images of ROS production using DHE staining with Hoechst in the NAc of rats repeatedly administered saline or nicotine on day 7 (E). *P < 0.05 and **P < 0.01 vs. the saline group. Bar = 100 μm. n = 3 ~ 4/group. F–H. Representative blots of oxidative stress markers (F), SOD-1 (G) and GPx-1/2 (H) in the NAc of rats repeatedly administered saline or nicotine on day 7. *P < 0.05 vs. the saline group. n = 4/group. All the values are presented as the mean ± SD.
