Fig. 2

Effect of PBN, a nonspecific ROS scavenger, on nicotine-induced hyperlocomotor activity in rats. (A). Experimental schedule. Saline or PBN (50 or 75 mg/kg, i.p.) was injected 10 min before saline or nicotine (0.4 mg/kg, s.c.) administration on day 7. B, C. Representative locomotor trajectories (red lines in each gray rectangle, (B)) and mean total distance traveled over 60 min after systemic injection of saline or nicotine on day 7 (C). (D). Temporal analysis of the total distance traveled at different times after saline or nicotine administration on day 7. ***P < 0.001 vs. the Sal + Sal group, # P < 0.05 or ## P < 0.01 vs. the Sal + Nic group. Sal = saline, Nic = nicotine. N = 5–9/group. All the values are presented as the mean ± SD.