Fig. 5 | Scientific Reports

Fig. 5

From: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

Fig. 5

Schematic representation of IVF using sperm bound to glycans pre-fertilization (glycan-IVF). Functionalized streptavidin-coverslips were coated with suLeX in droplets of medium. Sperm were added to the droplets and incubated to allow them to bind suLeX. The medium in the droplet was replaced to remove free sperm. Mature cumulus-oocyte complexes (COCs) were added to the droplet and co-cultured with bound sperm. COCs induce the release of some of the sperm enabling them to fertilize the oocytes.

Back to article page